Human CD30 Protein, His tag
|Package||10 μg ￥800.00 ; 50 μg ￥3100.00 ; 100 μg ￥4600.00|
|Description||Recombinant human CD30 protein with C-terminal 6×His tag|
|Tag||C- 6×His tag|
|Molecular Weight||The protein has a predicted molecular mass of 39.3 kDa after removal of the signal peptide. The apparent molecular mass of CD30-His is approximately 55-70 kDa due to glycosylation.|
|Purity||The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.|
|Formulation & Reconstitution||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.|
|Storage||Store at -80°C for 12 months (Avoid repeated freezing and thawing)|
|Usage||Research use only|
Figure 1. Human CD30 Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD30, His tagged protein (PME100481) can bind Anti-CD30 Neutralizing antibody (BME100017) in a linear range of 0.13-80.0 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD30, His tagged protein (PME100481) can bind Human CD30 Ligand,mFc-His tagged protein (PME100041) in a linear range of 2.56-320 ng/ml.
Figure 4. Flow cytometry analysis with 1 μg/mL Human CD30 Protein, His tag (PME100481) on Expi293 cells transfected with human CD30L (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
|Background||The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.|