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Human CD30 Protein, His tag

产品概述
Catalog Number PME100481
Package 10 μg ¥800.00 ; 50 μg ¥3100.00 ; 100 μg ¥4600.00
Description Recombinant human CD30 protein with C-terminal 6×His tag
Synonyms TNFRSF8,CD30,D1S166E,Ki-1
Delivery In Stock
Uniprot ID P28908
Expression Host HEK293
Tag C- 6×His tag
Molecular Characterization 这里上传图片
Molecular Weight The protein has a predicted molecular mass of 39.3 kDa after removal of the signal peptide. The apparent molecular mass of CD30-His is approximately 55-70 kDa due to glycosylation.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Storage Store at -80°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

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Figure 1. Human CD30 Protein, His Tag on SDS-PAGE under reducing condition.

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Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD30, His tagged protein (PME100481) can bind Anti-CD30 Neutralizing antibody (BME100017) in a linear range of 0.13-80.0 ng/ml.

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Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD30, His tagged protein (PME100481) can bind Human CD30 Ligand,mFc-His tagged protein (PME100041) in a linear range of 2.56-320 ng/ml.

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Figure 4. Flow cytometry analysis with 1 μg/mL Human CD30 Protein, His tag (PME100481) on Expi293 cells transfected with human CD30L (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
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