Anti-CD22 antibody(DM13), Rabbit mAb
Recombinant human CD22 (Asp20-Arg687) (PME100005) produced by using human HEK293 cells
|Buffer||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.|
|Package||10 μg ￥600.00 ; 100 μg ￥3880.00|
|IgG type||Rabbit IgG|
|Purification||Purified from cell culture supernatant by affinity chromatography|
|Applications||ELISA, Flow Cyt,WB, IP, IF|
|Recommended Dilutions||Flow Cyt 1/100|
|Storage||Store at -20°C for 12 months (Avoid repeated freezing and thawing)|
|Usage||Research use only|
Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD22 (right) were surface stained with Rabbit anti-CD22 monoclonal antibody 1μg/ml (clone: DM13) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD22 monoclonal antibody (clone: DM13) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 3. Affinity ranking of different Rabbit anti-CD22 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CD22 mAb clones. A) Heavy chain and B) Light chain.
|Background||CD22 (CD22 Molecule) is a Protein Coding gene. Diseases associated with CD22 include Refractory Hematologic Cancer and Hairy Cell Leukemia. Among its related pathways are Downstream signaling events of B Cell Receptor (BCR) and Hematopoietic cell lineage. Gene Ontology (GO) annotations related to this gene include carbohydrate binding. An important paralog of this gene is SIGLEC2.|