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Anti-CD22 antibody(DM13), Rabbit mAb

产品概述
Clone ID DM13
Immunogen Recombinant human CD22 (Asp20-Arg687) (PME100005) produced by using human HEK293 cells
Buffer Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Catalog Number DME100013
Package 10 μg ¥600.00 ; 100 μg ¥3880.00
Delivery In Stock
Synonyms SIGLEC-2, SIGLEC2
Host Species Rabbit
IgG type Rabbit IgG
Clonality Monoclonal
Formulation Powder
Purification Purified from cell culture supernatant by affinity chromatography
Reactivity Human
Applications ELISA, Flow Cyt,WB, IP, IF
Recommended Dilutions Flow Cyt  1/100
Storage Store at -20°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

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Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD22 (right) were surface stained with Rabbit anti-CD22 monoclonal antibody 1μg/ml (clone: DM13) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

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Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD22 monoclonal antibody (clone: DM13) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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Figure 3. Affinity ranking of different Rabbit anti-CD22 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CD22 mAb clones. A) Heavy chain and B) Light chain.

Background CD22 (CD22 Molecule) is a Protein Coding gene. Diseases associated with CD22 include Refractory Hematologic Cancer and Hairy Cell Leukemia. Among its related pathways are Downstream signaling events of B Cell Receptor (BCR) and Hematopoietic cell lineage. Gene Ontology (GO) annotations related to this gene include carbohydrate binding. An important paralog of this gene is SIGLEC2.
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