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Anti-SARS-CoV-2 Nucleocapsid antibody(DM23), Rabbit mAb

产品概述
Clone ID DM23
Immunogen Recombinant SARS-CoV-2 Nucleocapsid (Met1-Ala 419) (PME100459) produced by using E. coli
Buffer Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Catalog Number DME100016
Package 10 μg ¥600.00 ; 100 μg ¥3180.00
Delivery In Stock
Synonyms Nucleocapsid, N protein, protein N,SARS-CoV-2,SARS
Host Species Rabbit
IgG type Rabbit IgG
Clonality Monoclonal
Formulation Powder
Purification Purified from cell culture supernatant by affinity chromatography
Reactivity SARS-CoV-2
Applications ELISA
Recommended Dilutions ELISA 1/5000-1/10000
Storage Store at -20°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

Figure 1. Elisa plate pre-coated by 2 μg/ml(100μl/well) SARS-CoV-2 Nucleocapsid protein, His Tag(Cat.No.PME100459) can bind Rabbit Anti-SARS-CoV-2 Nucleocapsid monoclonal antibody (clone:DM23) in a linear range of 0.24-62.5 ng/ml.

Background Coronavirus contain most of nucleocapsid protein. Coronavirus nucleoproteins (N proteins) localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The nucleolus is the site of ribosome biogenesis and sequesters cell cycle regulatory complexes. Two of the major components of the nucleolus are fibrillarin and nucleolin. These proteins are involved in nucleolar assembly and ribosome biogenesis and act as chaperones for the import of proteins into the nucleolus. Regarding of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is a tool for diagnostic.
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