Human IL5 Protein, His Tag
|Package||10 μg ￥750.00 ; 50 μg ￥2900.00 ; 100 μg ￥4400.00|
|Description||Recombinant Human IL5 protein with C-terminal 6×His tag|
|Synonyms||IL-5, TRF, Interleukin-5, EDF|
|Molecular Weight||The protein has a predicted molecular mass of 13.94 kDa after removal of the signal peptide.The apparent molecular mass of IL5-His is approximately 15-25 kDa due to glycosylation.|
|Purity||The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.|
|Formulation & Reconstitution||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.|
|Storage||Store at -80°C for 12 months (Avoid repeated freezing and thawing)|
|Usage||Research use only|
Figure 1. Human IL5 Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. Flow cytometry analysis with 1 μg/mL Human IL5 Protein, His Tag (PME100085) on Expi293 cells transfected with Human IL5RA protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
|Background||This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.|