Human ACE2 Protein, His Tag
|Package||10 μg ￥650.00 ; 50 μg ￥2600.00 ; 100 μg ￥3900.00|
|Description||Recombinant Human ACE2 Protein with N-terminal 6×His tag|
|Molecular Weight||The protein has a predicted molecular mass of 84.4 kDa after removal of the signal peptide. The apparent molecular mass of His-ACE2 is approximately 100-130 kDa due to glycosylation.|
|Purity||The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.|
|Formulation & Reconstitution||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.|
|Storage||Store at -80°C for 12 months (Avoid repeated freezing and thawing)|
|Usage||Research use only|
Figure 1. Human ACE2 Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human ACE2, His Tagged protein (PME100490) can bind S-RBD, hFc tagged protein (PME100487) in a linear range of 0.128-80.0 ng/ml.
|Background||The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.|