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Human ACE2 Protein, His Tag

产品概述
Catalog Number PME100490
Package 10 μg ¥600.00 ; 50 μg ¥2450.00 ; 100 μg ¥3680.00
Description Recombinant Human ACE2 Protein with N-terminal 6×His tag
Synonyms ACE-2,ACEH,ACE2
Uniprot ID Q9BYF1
Expression Host HEK293
Tag N-6×His tag
Molecular Characterization 这里上传图片
Molecular Weight The protein has a predicted molecular mass of 84.4 kDa after removal of the signal peptide. The apparent molecular mass of His-ACE2 is approximately 10-130 kDa due to glycosylation.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
Storage Store at -80°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

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Figure 1. Human ACE2 Protein, His Tag on SDS-PAGE under reducing condition.

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Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human ACE2, His Tagged protein(PME100490)can bind S-RBD, hFc tagged protein (PME100487) in a linear range of 0.128-80.0 ng/ml.

Background The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
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