Anti-CD27 antibody(DM59), Rabbit mAb
Recombinant human CD27 (Ala20-Arg191) (PME100475) produced by using human HEK293 cells
|Buffer||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.|
|Package||10 μg ￥600.00 ; 100 μg ￥3880.00|
|Synonyms||CD27, TNFRSF7, S152, T14, Tp55|
|IgG type||Rabbit IgG|
|Purification||Purified from cell culture supernatant by affinity chromatography|
|Recommended Dilutions||ELISA 1/5000-10000;Flow Cyt 1/100|
|Storage||Store at -20°C for 12 months (Avoid repeated freezing and thawing)|
|Usage||Research use only|
Figure 1. Expi 293 cell line transfected with irrelevant protein (A) and human CD27 (B) were surface stained with Rabbit anti-CD27 monoclonal antibody 1μg/ml (clone: DM59) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD27 monoclonal antibody (clone: DM59) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 3. Affinity ranking of different Rabbit anti-CD27 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
|Background||The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.|