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Anti-GPRC5D antibody(DM60), Rabbit mAb

产品概述
Clone ID DM60
Immunogen Recombinant human GPRC5D (Thr1-Pro21) (PME100066) produced by using human HEK293 cells
Buffer Preservative: 0.1% Procline 300
Constituents: 50% Glycerol; PBS,pH 7.4; 0.1% BSA
Catalog Number DME100060
Package 10 μl ¥600.00 ; 100 μl ¥3880.00
Synonyms GPRC5D
Host Species Rabbit
IgG type Rabbit IgG
Clonality Monoclonal
Formulation Liquid
Purification Purified from cell culture supernatant by affinity chromatography
Reactivity Human
Applications ELISA,Flow Cyt
Recommended Dilutions ELISA 1/5000-10000;Flow Cyt  1/100
Storage Store at -20°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

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Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human GPRC5D protein, hFc-His tagged protein (PME100066) can bind Rabbit anti-GPRC5D monoclonal antibody (clone: DM60) in a linear range of 1-100 ng/ml.

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Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human GPRC5D (B) were surface stained with Rabbit anti-GPRC5D monoclonal antibody 15μg/ml (clone: DM60)followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

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Figure 3. Flow cytometry data of serially titrated Rabbit anti- GPRC5D monoclonal antibody (clone: DM60) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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Figure 4. Affinity ranking of different Rabbit anti-GPRC5D mAb clones by titration of different concentration onto H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Background The protein encoded by this gene is a member of the G protein-coupled receptor family; however, the specific function of this gene has not yet been determined.
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