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Anti-GPRC5D antibody(DM61), Rabbit mAb

产品概述
Clone ID DM61
Immunogen Recombinant human GPRC5D (Thr1-Pro21) (PME100066) produced by using human HEK293 cells
Buffer Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Catalog Number DME100061
Package 10 μg ¥600.00 ; 100 μg ¥3880.00
Delivery In Stock
Synonyms GPRC5D
Host Species Rabbit
IgG type Rabbit IgG
Clonality Monoclonal
Formulation Powder
Purification Purified from cell culture supernatant by affinity chromatography
Reactivity Human
Applications ELISA,Flow Cyt
Recommended Dilutions ELISA 1/5000-10000;Flow Cyt  1/100
Storage Store at -20°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

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Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human GPRC5D protein, hFc-His tagged protein (PME100066) can bind Rabbit anti-GPRC5D monoclonal antibody (clone: DM61) in a linear range of 1-100 ng/ml.

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Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human GPRC5D (B) were surface stained with Rabbit anti-GPRC5D monoclonal antibody 15μg/ml (clone: DM61) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

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Figure 3. Flow cytometry data of serially titrated Rabbit anti- GPRC5D monoclonal antibody (clone: DM61) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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Figure 4. Affinity ranking of different Rabbit anti-GPRC5D mAb clones by titration of different concentration onto H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Background The protein encoded by this gene is a member of the G protein-coupled receptor family; however, the specific function of this gene has not yet been determined.
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