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Anti-B7-H2 antibody(DM98), Rabbit mAb

产品概述
Clone ID DM98
Immunogen Recombinant human B7-H2 (Asp19-Thr256) (PME100029) produced by using human HEK293 cells
Buffer Preservative: 0.1% Procline 300
Constituents: 50% Glycerol; PBS,pH 7.4; 0.1% BSA
Catalog Number DME100098
Package 10 μl ¥600.00 ; 100 μl ¥3880.00
Synonyms ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, LICOS, ICOS ligand
Host Species Rabbit
IgG type Rabbit IgG
Clonality Monoclonal
Formulation Liquid
Purification Purified from cell culture supernatant by affinity chromatography
Reactivity Human
Applications ELISA,Flow Cyt
Recommended Dilutions ELISA 1/5000-10000;Flow Cyt  1/100
Storage Store at -20°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

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Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human B7-H2 protein, mFc-His tagged protein (PME100029) can bind Rabbit anti-B7-H2 monoclonal antibody (clone: DM98) in a linear range of 3.2-80 ng/ml.

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Figure 2. Flow cytometry analysis with Anti-B7-H2 (DM98) on Expi293 cells transfected with human B7-H2 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Background Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2.The protein is the ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
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