Anti-B7-H2 antibody(DM99), Rabbit mAb
Recombinant human B7-H2 (Asp19-Thr256) (PME100029) produced by using human HEK293 cells
|Buffer||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.|
|Package||10 μg ￥600.00 ; 100 μg ￥3880.00|
|Synonyms||ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, LICOS, ICOS ligand|
|IgG type||Rabbit IgG|
|Purification||Purified from cell culture supernatant by affinity chromatography|
|Recommended Dilutions||ELISA 1/5000-10000;Flow Cyt 1/100|
|Storage||Store at -20°C for 12 months (Avoid repeated freezing and thawing)|
|Usage||Research use only|
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human B7-H2 protein, mFc-His tagged protein (PME100029) can bind Rabbit anti-B7-H2 monoclonal antibody (clone: DM99) in a linear range of 3.2-80 ng/ml.
Figure 2. Flow cytometry analysis with Anti-B7-H2 (DM99) on Expi293 cells transfected with human B7-H2 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
|Background||Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2.The protein is the ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.|