Anti-CD40 antibody(DM101), Rabbit mAb
Recombinant human CD40 (Glu21-Arg193) (PME100015) produced by using human HEK293 cells
|Buffer||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.|
|Package||10 μg ￥600.00 ; 100 μg ￥3880.00|
|Synonyms||CD40, Bp50, CDW40, MGC9013, TNFRSF5, p50|
|IgG type||Rabbit IgG|
|Purification||Purified from cell culture supernatant by affinity chromatography|
|Recommended Dilutions||ELISA 1/5000-10000;Flow Cyt 1/100|
|Storage||Store at -20°C for 12 months (Avoid repeated freezing and thawing)|
|Usage||Research use only|
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD40 protein, mFc-His tagged protein (PME100015) can bind Rabbit anti-CD40 monoclonal antibody (clone: DM101) in a linear range of 0.64-80 ng/ml.
Figure 2. Flow cytometry analysis with Anti-CD40 (DM101) on Expi293 cells transfected with human CD40 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
|Background||This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.|