Human PD-1 Protein, hFc-His tag
|Package||10 μg ￥800.00 ; 50 μg ￥3100.00 ; 100 μg ￥4600.00|
|Description||Recombinant human PD-1 protein with C-terminal human Fc and 6×His tag|
|Synonyms||PDCD1, PD1, CD279, SLEB2|
|Tag||C-Human Fc and 6×His tag|
|Molecular Weight||The protein has a predicted molecular mass of 42.9 kDa after removal of the signal peptide.|
|Purity||The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.|
|Formulation & Reconstitution||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.|
|Storage||Store at -80°C for 12 months (Avoid repeated freezing and thawing)|
|Usage||Research use only|
Figure 1. Human PD-1 Protein, hFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human PD1, hFc-His tagged protein (PME100462) can bind Human PDL1, mFc-His tagged protein (PME100023) in a linear range of 62.5-251.1 ng/ml.
Figure 3. Flow cytometry analysis with 0.2 μg/ml Human PD-1 Protein, hFc-His tag (PME100462) on Expi293 cells transfected with human PD-L1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 4. HEK293 cell line transfected with irrelevant protein (red histogram) and human PD-L2 protein (blue histogram) were surface stained with 2ug/ml Human PD-1 Protein, hFc-His tag (PME100462)followed by PE-conjugated Goat anti-human IgG secondary antibody.
|Background||This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.|