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DIMA recombinant extra-cellular domain (ECD) fusion protein expression system

Currently almost all the therapeutic antibody drugs target on extra-cellular domain of human membrane proteins or secreted protein molecules. To express and purify whole length membrane proteins in native conformation sometimes are very challenging or impossible. The creation of ECD-Fc fusion protein is a commonly used approach to overcome this issue. For druggable targets, DIMA will construct ECD-Fc fusion protein and use HEK293 mammalian expression system for production. This will not only ensure the authentic posttranslational modifications from mammalian cells, but also have a high chance of obtaining proteins in good solubility and correct folding under standard buffer system.

Platform Flow
Key advantages

1. Mammalian expression system with authentic posttranslational modifications
2. Serum free medium culturing system: minimize host cell contamination
3. ECD-Fc fusion with improved stability and solubility in standard aqueous buffer
4. Custom protein and large batch production available

Key applications with DIMA purified proteins

1. Native immunogens for therapeutic antibody drug development
2. Native ligand and receptor identification
3. Therapeutic drug In Vitro functional test
4. In Vitro protein functional test
5. Cell based assays