Human CD70 Protein, mFc-His Tag
|Package||10 μg $80.00 ; 50 μg $310.00 ; 100 μg $460.00|
|Description||Recombinant human CD70 protein with N-terminal mouse Fc and 6×His tag|
|Tag||N-Mouse Fc and C-6×His tag|
|Molecular Weight||The protein has a predicted molecular mass of 45.6 kDa after removal of the signal peptide.|
|Purity||The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.|
|Formulation & Reconstitution||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 46% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.|
|Storage||Store at -80°C for 12 months (Avoid repeated freezing and thawing)|
|Usage||Research use only|
Figure 1. Human CD70 Protein, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD27, hFc tagged protein (PME100475) can bind Human CD70,mFc-His tagged protein (PME100042) in a linear range of 3.2-400 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) CD70,mFc-His tagged protein (PME100042) can bind Anti-CD70 Neutralizing antibody (BME100005) in a linear range of 3.91-139.9 ng/ml.
Figure 4. Flow cytometry analysis with 15 μg/mL Human CD70 Protein, mFc-His tag (PME100042) on Expi293 cells transfected with human CD27 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
|Background||The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis.|