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Human CD70 Protein, mFc-His Tag

Product Overview
Catalog Number PME100042
Package 10 μg $60.00 ; 50 μg $235.00 ; 100 μg $338.00
Description Recombinant human CD70 protein with N-terminal mouse Fc and 6×His tag
Synonyms CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
Uniprot ID P32970
Expression Host HEK293
Tag N-Mouse Fc and C-6×His tag
Molecular Characterization 这里上传图片
Molecular Weight The protein has a predicted molecular mass of 45.6 kDa after removal of the signal peptide.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
Storage Store at -80°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

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Figure 1. Human CD70 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

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Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD27, hFc tagged protein (PME100475) can bind Human CD70,mFc-His tagged protein(PME100042) in a linear range of 3.2-400 ng/ml.

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Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) CD70,mFc-His tagged protein(PME100042) can bind Anti-CD70 Neutralizing antibody (BME100005) in a linear range of 3.91-139.9 ng/ml.

Background The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis.
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