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Human CD27 Protein, hFc Tag

Product Overview
Catalog Number PME100475
Package 10 μg $80.00 ; 50 μg $310.00 ; 100 μg $460.00
Description Recombinant human CD27 protein with C-terminal human Fc tag
Synonyms CD27, TNFRSF7, S152, T14, Tp55
Delivery In Stock
Uniprot ID P26842
Expression Host HEK293
Tag C-Human Fc
Molecular Characterization 这里上传图片
Molecular Weight The protein has a predicted molecular mass of 45.3 kDa after removal of the signal peptide.The apparent molecular mass of CD27-hFc is approximately 55-70 kDa due to glycosylation.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Storage Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized proteins are shipped at ambient temperature.
Usage Research use only


Figure 1. Human CD27 Protein, hFc Tag on SDS-PAGE under reducing condition.


Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD27, hFc tagged protein (PME100475) can bind Anti-CD27 Neutralizing antibody (BME100018) in a linear range of 3.2-80.0 ng/ml.


Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD27, hFc tagged protein (PME100475) can bind Human CD70,mFc-His tagged protein (PME100042) in a linear range of 3.2-400 ng/ml.


Figure 4. Flow cytometry analysis with 15 μg/mL Human CD27 Protein, hFc tag (PME100475) on Expi293 cells transfected with human CD70 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
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