Human CD27 Protein, hFc Tag
|Package||10 μg $80.00 ; 50 μg $310.00 ; 100 μg $460.00|
|Description||Recombinant human CD27 protein with C-terminal human Fc tag|
|Synonyms||CD27, TNFRSF7, S152, T14, Tp55|
|Molecular Weight||The protein has a predicted molecular mass of 45.3 kDa after removal of the signal peptide.The apparent molecular mass of CD27-hFc is approximately 55-70 kDa due to glycosylation.|
|Purity||The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.|
|Formulation & Reconstitution||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.|
|Storage||Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).|
|Usage||Research use only|
Figure 1. Human CD27 Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD27, hFc tagged protein (PME100475) can bind Anti-CD27 Neutralizing antibody (BME100018) in a linear range of 3.2-80.0 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD27, hFc tagged protein (PME100475) can bind Human CD70,mFc-His tagged protein (PME100042) in a linear range of 3.2-400 ng/ml.
Figure 4. Flow cytometry analysis with 15 μg/mL Human CD27 Protein, hFc tag (PME100475) on Expi293 cells transfected with human CD70 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
|Background||The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.|