Human EGFR Protein, His Tag
|Package||10 μg $60.00 ; 50 μg $235.00 ; 100 μg $338.00|
|Description||Recombinant human EGFR Protein with C-6×His tag|
|Molecular Weight||The protein has a predicted molecular mass of 69.4 kDa after removal of the signal peptide.The apparent molecular mass of EGFR-His is approximately 100-130 kDa due to glycosylation.|
|Purity||The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.|
|Formulation & Reconstitution||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.|
|Storage||Store at -80°C for 12 months (Avoid repeated freezing and thawing)|
|Usage||Research use only|
Figure 1. Human EGFR Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human EGFR, His tagged protein (PME100099) can bind Anti-EGFR Antibody (BME100034) in a linear range of 0.64-80.0 ng/ml.
|Background||The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.|