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Human EGFR Protein, His Tag

Product Overview
Catalog Number PME100099
Package 10 μg $60.00 ; 50 μg $235.00 ; 100 μg $338.00
Description Recombinant human EGFR Protein with C-6×His tag
Synonyms EGFR,ERBB,ERBB1,HER1,PIG61,mENA
Uniprot ID P00533
Expression Host HEK293
Tag C-6×His tag
Molecular Characterization 这里上传图片
Molecular Weight The protein has a predicted molecular mass of 69.4 kDa after removal of the signal peptide.The apparent molecular mass of EGFR-His is approximately 100-130 kDa due to glycosylation.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
Storage Store at -80°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

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Figure 1. Human EGFR Protein, His Tag on SDS-PAGE under reducing condition.

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Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human EGFR, His tagged protein (PME100099) can bind Anti-EGFR Antibody (BME100034) in a linear range of 0.64-80.0 ng/ml.


Background The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
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