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Anti-CD28 antibody(DM64), Rabbit mAb

Product Overview
Clone ID DM64
Immunogen Recombinant human CD28 (Asn19-Pro152) (PME100014) produced by using human HEK293 cells
Buffer Preservative: 0.1% Procline 300
Constituents: 50% Glycerol; PBS,pH 7.4; 0.1% BSA
Catalog Number DME100064
Package 10 μl $60.00 ; 100 μl $398.00
Synonyms CD28, Tp44
Host Species Rabbit
IgG type Rabbit IgG
Clonality Monoclonal
Formulation Liquid
Purification Purified from cell culture supernatant by affinity chromatography
Reactivity Human
Applications ELISA,Flow Cyt
Recommended Dilutions ELISA 1/5000-10000;Flow Cyt  1/100
Storage Store at -20°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

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Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD28 protein, mFc-His tagged protein (PME100014) can bind Rabbit anti-CD28 monoclonal antibody (clone: DM64) in a linear range of 1-100 ng/ml.

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Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human CD28 (B) were surface stained with Rabbit anti-CD28 monoclonal antibody 1μg/ml (clone: DM64) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

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Figure 3. Flow cytometry data of serially titrated Rabbit anti-CD28 monoclonal antibody (clone: DM64) on Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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Figure 4. Affinity ranking of different Rabbit anti- CD28 mAb clones by titration of different concentration onto Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Background The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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