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Anti-SARS-CoV-2 RBD antibody(DM54), Rabbit mAb

Product Overview
Clone ID DM54
Immunogen Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD (PME100497) (Arg 319-Phe541) produced by using human HEK293 cells
Buffer Preservative: 0.1% Procline 300
Constituents: 50% Glycerol; PBS, pH 7.4; 0.1% BSA
Catalog Number DME100054
Package 10 μl $60.00 ; 100 μl $318.00
Synonyms SARS-CoV-2 RBD
Host Species Rabbit
IgG type Rabbit IgG
Clonality Monoclonal
Formulation Liquid
Purification Purified from cell culture supernatant by affinity chromatography
Reactivity SARS-CoV-2
Applications ELISA,Flow Cyt
Recommended Dilutions ELISA 1/5000-10000;Flow Cyt  1/100
Storage Store at -20°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only


Figure 1. Elisa plate pre-coated by 2 μg/ml (100μl/well) SARS-CoV-2 RBD protein can bind Rabbit Anti-SARS-CoV-2 RBD monoclonal antibody (clone:DM54) in a linear range of 0.1-100 ng/ml.


Figure 2. Competition flow cytometry assay demonstrating Rabbit anti-RBD monoclonal antibody (clone: DM54) blockade of SARS-CoV-2 (COVID-19) S protein RBD (1μg/ml, PME100497) binding to Expi 293 cell line transfected with human ACE2. IC50=816.4ng/ml. The Y-axis represents the geometric mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Background SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.
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