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Anti-CD138 antibody(DM56), Rabbit mAb

Product Overview
Clone ID DM56
Immunogen Recombinant human CD138 (Gln23-Gly254) (PME100006) produced by using human HEK293 cells
Buffer
Preservative: 0.1% Procline 300
Constituents: 50% Glycerol; PBS,pH 7.4; 0.1% BSA
Catalog Number DME100056
Package 10 μl $60.00 ; 100 μl $398.00
Synonyms SDC1, Syndecan-1, CD138, SYND1, SDC
Host Species Rabbit
IgG type Rabbit IgG
Clonality Monoclonal
Formulation Liquid
Purification Purified from cell culture supernatant by affinity chromatography
Reactivity Human
Applications ELISA,Flow Cyt
Recommended Dilutions ELISA 1/5000-10000;Flow Cyt  1/100
Storage Store at -20°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

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Figure 1. Expi 293 cell line transfected with irrelevant protein (A) and human CD138 (B) were surface stained with Rabbit anti-CD138 monoclonal antibody 1μg/ml (clone: DM56) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

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Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD138 monoclonal antibody (clone: DM56) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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Figure 3. Affinity ranking of different Rabbit anti-CD138 mAb clones by titration of different concentration onto H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Background Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138, is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 / SDC1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells, but only if the cells tested are already known to be derived from blood.
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