Anti-41BB Ligand antibody(DM68), Rabbit mAb
Recombinant human 4-1BB Ligand (Pro52-Glu254) (PME100054) produced by using human HEK293 cells
Preservative: 0.1% Procline 300
Constituents: 50% Glycerol; PBS, pH 7.4; 0.1% BSA
|Package||10 μl $60.00 ; 100 μl $398.00|
|IgG type||Rabbit IgG|
|Purification||Purified from cell culture supernatant by affinity chromatography|
|Recommended Dilutions||ELISA 1/5000-10000;Flow Cyt 1/100|
|Storage||Store at -20°C for 12 months (Avoid repeated freezing and thawing)|
|Usage||Research use only|
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human 4-1BB Ligand protein, mFc-His tagged protein (PME100054) can bind Rabbit anti-4-1BB Ligand monoclonal antibody (clone: DM68) in a linear range of 1-100 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human 4-1BB Ligand (B) were surface stained with Rabbit anti-4-1BB Ligand monoclonal antibody 1μg/ml (clone: DM68) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry data of serially titrated Rabbit anti-4-1BB Ligand monoclonal antibody (clone: DM68) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
|Background||The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.|