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Anti-2B4 antibody(DM69), Rabbit mAb

Product Overview
Clone ID DM69
Immunogen Recombinant human 2B4 (Cys22-Ala221) (PME100010) produced by using human HEK293 cells
Buffer Preservative: 0.1% Procline 300
Constituents: 50% Glycerol; PBS,pH 7.4; 0.1% BSA
Catalog Number DME100069
Package 10 μl $60.00 ; 100 μl $398.00
Synonyms CD244,2B4,SLAMF4,NKR2B4,NAIL,h2B4
Host Species Rabbit
IgG type Rabbit IgG
Clonality Monoclonal
Formulation Liquid
Purification Purified from cell culture supernatant by affinity chromatography
Reactivity Human
Applications ELISA,Flow Cyt
Recommended Dilutions ELISA 1/5000-10000;Flow Cyt  1/100
Storage Store at -20°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

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Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human 2B4 protein, mFc-His tagged protein (PME100010) can bind Rabbit anti-2B4 monoclonal antibody (clone: DM69) in a linear range of 1-100 ng/ml.

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Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human 2B4 (B) were surface stained with Rabbit anti-2B4 monoclonal antibody 1μg/ml (clone: DM69) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

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Figure 3. Flow cytometry data of serially titrated Rabbit anti-2B4 monoclonal antibody (clone: DM69) on THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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Figure 4. Affinity ranking of different Rabbit anti-2B4 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Background This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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