Anti-2B4 antibody(DM69), Rabbit mAb
| Clone ID |
DM69 |
|---|---|
| Immunogen |
Recombinant human 2B4 (Cys22-Ala221) (PME100010) produced by using human HEK293 cells |
| Buffer |
Preservative: 0.1% Procline 300 Constituents: 50% Glycerol; PBS,pH 7.4; 0.1% BSA |
| Catalog Number | DME100069 |
|---|---|
| Package | 10 μl $60.00 ; 100 μl $398.00 |
| Synonyms | CD244,2B4,SLAMF4,NKR2B4,NAIL,h2B4 |
| Host Species | Rabbit |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Formulation | Liquid |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Reactivity | Human |
| Applications | ELISA,Flow Cyt |
| Recommended Dilutions | ELISA 1/5000-10000;Flow Cyt 1/100 |
| Storage | Store at -20°C for 12 months (Avoid repeated freezing and thawing) |
| Usage | Research use only |
| Images |
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human 2B4 protein, mFc-His tagged protein (PME100010) can bind Rabbit anti-2B4 monoclonal antibody (clone: DM69) in a linear range of 1-100 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human 2B4 (B) were surface stained with Rabbit anti-2B4 monoclonal antibody 1μg/ml (clone: DM69) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry data of serially titrated Rabbit anti-2B4 monoclonal antibody (clone: DM69) on THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Affinity ranking of different Rabbit anti-2B4 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
|
| Background | This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Datasheet | Download | COA | Download |
|---|---|---|---|
| MSDS | Download | ||
简体中文
English
