Human CD123 Protein, hFc-His Tag
|Package||10 μg $60.00 ; 50 μg $245.00 ; 100 μg $358.00|
|Description||Recombinant human CD123 protein with C-terminal human Fc and 6×His tag|
|Synonyms||IL3R, IL3RA, IL-3Ra, IL-3R-alpha, IL3RAY, IL3RX, IL3RY, CD123 antigen, CD123, hIL3Ra, hIL-3Ra, MGC34174, IL-3 R alpha|
|Tag||C-Human Fc and 6×His tag|
|Molecular Weight||The protein has a predicted molecular mass of 80-90 kDa after removal of the signal peptide.|
|Purity||The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.|
|Formulation & Reconstitution||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.|
|Storage||Store at -80°C for 12 months (Avoid repeated freezing and thawing)|
|Usage||Research use only|
Figure 1. Human CD123, hFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD123, hFc-His tagged protein (PME100003) can bind Anti-CD123 Neutralizing antibody (BME100003) in a linear range of 0.98-26.70 ng/ml.
|Background||Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells.|