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SARS-CoV-2 (2019-nCoV) S protein RBD, His Tag

Product Overview
Catalog Number PME100036
Description Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD with 6×His tag
Synonyms S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD, COVID-19
Expression Host HEK293
Tag C-6×His tag
Molecular Characterization 这里上传图片
Molecular Weight The protein has a predicted molecular mass of 37-38 kDa after removal of the signal peptide.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Buffer Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 23 % trehalose is added as protectants before lyophilization.
Storage Store at -80°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

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Figure 1. SARS-CoV-2 (2019-nCoV) S protein RBD, His Tag on SDS-PAGE under reducing condition.

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Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) SARS-CoV-2 (2019-nCoV) S protein RBD, His tagged protein (PME100036) can bind its native ligand Human ACE2, hFc tagged protein in a linear range of 1.96-31.72 ng/ml.

Background SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.
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