Human 2B4 Protein, mFc-His Tag
|Package||10 μg $60.00 ; 50 μg $245.00 ; 100 μg $358.00|
|Description||Recombinant human 2B4 protein with C-terminal mouse Fc and 6×His tag|
|Synonyms||CD244, 2B4, SLAMF4, NKR2B4, NAIL, h2B4|
|Tag||C-Mouse Fc and 6×His tag|
|Molecular Weight||The protein has a predicted molecular mass of 48.8 kDa after removal of the signal peptide. The apparent molecular mass of 2B4-mFc-His is approximately 70-100 kDa due to glycosylation.|
|Purity||The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.|
|Formulation & Reconstitution||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.|
|Storage||Store at -80°C for 12 months (Avoid repeated freezing and thawing)|
|Usage||Research use only|
Figure 1. Human 2B4, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human 2B4, mFc-His tagged protein (PME100010) can bind Human CD48, hFc tagged protein (PME100476) in a linear range of 31.25-4000 ng/ml.
|Background||This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.|