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Human 2B4 Protein, mFc-His Tag

Product Overview
Catalog Number PME100010
Package 10 μg $60.00 ; 50 μg $245.00 ; 100 μg $358.00
Description Recombinant human 2B4 protein with C-terminal mouse Fc and 6×His tag
Synonyms CD244, 2B4, SLAMF4, NKR2B4, NAIL, h2B4
Expression Host HEK293
Tag C-Mouse Fc and 6×His tag
Molecular Characterization 这里上传图片
Molecular Weight The protein has a predicted molecular mass of 48.8 kDa after removal of the signal peptide. The apparent molecular mass of 2B4-mFc-His is approximately 70-100 kDa due to glycosylation.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
Storage Store at -80°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

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Figure 1. Human 2B4, mFc-His Tag on SDS-PAGE under reducing condition.

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Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human 2B4, mFc-His tagged protein (PME100010) can bind Human CD48, hFc tagged protein (PME100476) in a linear range of 31.25-4000 ng/ml.

Background This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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