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Human PD-1 Protein, mFc-His tag

Product Overview
Catalog Number PME100025
Package 10 μg $80.00 ; 50 μg $310.00 ; 100 μg $460.00
Description Recombinant human PD-1 protein with C-terminal mouse Fc and 6×His tag
Synonyms PDCD1, PD1, CD279, SLEB2
Delivery In Stock
Uniprot ID Q15116
Expression Host HEK293
Tag C-Mouse Fc and 6×His tag
Molecular Characterization 这里上传图片
Molecular Weight The protein has a predicted molecular mass of 43.5 kDa after removal of the signal peptide.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Storage Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized proteins are shipped at ambient temperature.
Usage Research use only

Figure 1. Human PD-1 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human PD1, mFc-His tagged protein (PME100025) can bind Anti-PD-1 Neutralizing antibody (BME100006) in a linear range of 0.24-6.49 ng/ml.

Figure 3. Flow cytometry analysis with 2μg/ml Human PD-1 Protein, mFc-His tag (PME100025) on Expi293 cells transfected with human PD-L1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Figure 4. HEK293 cell line transfected with irrelevant protein (red histogram) and human PD-L2 protein (blue histogram) were surface stained with  2ug/ml Human PD-1 Protein, mFc-His tag (PME100025) followed by PE-conjugated Goat anti-mouse IgG secondary antibody.

Background This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
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