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Anti-His Tag Antibody(DM1), Rabbit mAb

Product Overview
Clone ID DM1
Immunogen 6×His synthetic peptide coupled to KLH
Buffer Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Catalog Number DME100001
Package 10 μg $50.00 ; 100 μg $268.00
Delivery In Stock
Synonyms his tag
Host Species Rabbit
IgG type IgG
Clonality Monoclonal
Formulation Liquid
Purification Purified from cell culture supernatant by affinity chromatography
Reactivity Human
Applications WB, ELISA
Recommended Dilutions WB 1/1000
Storage Store at -20°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only

Western blot analysis of his-tag fusion protein using His-Tag antibody (DME100001) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:5000 dilution.
His-tag fusion protein :  70kDa, 20ng per lane.
Blocking buffer: 5% nonfat dry milk in TBST.
Exposure time: 1s. 

Background The rabbit immune system generates antibody diversity and optimizes affinity. Anti-His Tag Antibody(DM1), Rabbit mAb is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteinsin bacteria, insect cells, and mammalian cells.
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