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Human CD40 Ligand Protein, hFc Tag

Product Overview
Catalog Number PME100192
Package 10 μg $70.00; 50 μg $280.00;100 μg $380.00
Description Recombinant human CD40 Ligand Protein with N-terminal Human Fc tag
Synonyms CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39
Delivery In Stock
Uniprot ID P29965
Expression Host HEK293
Tag N-Human Fc tag
Molecular Characterization 这里上传图片
Molecular Weight The protein has a predicted molecular mass of 42.3 kDa after removal of the signal peptide.The apparent molecular mass of hFc-CD40 Ligand is approximately 40-53 kDa due to glycosylation.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Storage Store at -80°C for 12 months (Avoid repeated freezing and thawing)
Usage Research use only
Images

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Figure 1. Human CD40 Ligand Protein, hFc Tag on SDS-PAGE under reducing condition.

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Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD40 Ligand,hFc tagged protein (PME100192) can bind Human CD40, mFc-His tagged protein (PME100015) in a linear range of 0.51-320 ng/ml.

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Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD40L, hFc tagged protein (PME100192) can bind Anti-CD40L Neutralizing antibody (BME100042) in a linear range of 0.64-400 ng/ml.

Background The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
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