ELISA, Flow Cyt
ELISA 1/5000-10000;Flow Cyt 1/100
Purified from cell culture supernatant by affinity chromatography
|Formulation & Reconstitution||
Research use only
The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Anti-CD28 antibody(DM64), Rabbit mAb
Price: 10μg $99.00 ; 100 μg $438.00 ; 500 μg $1314.00
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD28 protein, mFc-His tagged protein (PME100014) can bind Rabbit anti-CD28 monoclonal antibody (clone: DM64) in a linear range of 1-100 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human CD28 (B) were surface stained with Rabbit anti-CD28 monoclonal antibody 1μg/ml (clone: DM64) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry data of serially titrated Rabbit anti-CD28 monoclonal antibody (clone: DM64) on Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Affinity ranking of different Rabbit anti- CD28 mAb clones by titration of different concentration onto Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.