Extra Cellular Domain (ECD) Protein
Currently almost all the therapeutic antibody drugs target on extra-cellular domain of human membrane proteins or secreted protein molecules. It is challenging and sometimes impossible to express and purify full length transmembrane proteins in native conformation.
The ECD-Fc fusion protein is normally used to overcome this issue especially for the single transmembrane druggable targets.
DIMA constructed over 1000 expression plasmids for different ECD-Fc fusion proteins and used HEK293 mammalian expression system for production.
This not only ensure the authentic posttranslational modifications from mammalian cells, but also have a high chance of obtaining proteins in good solubility and correct folding under standard buffer system.