Biosimilar Reference Antibodies
To develop a superior biologic drug, it is important to synthesize a benchmark reference antibody. The reference antibody can greatly facilitate the downstream development. It can help identify critical cell lines, reagents, performance characteristics, etc.

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Active Protein Detection

Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human PD1, mFc-His tagged protein (PME100025) can bind Anti-PD-1 Neutralizing antibody in a linear range of 0.24-6.49 ng/ml.
Comparison of flow functional detection

Figure 2. HEK293 cell line transfected with irrelevant protein (B) and human PD1(A) were surface stained with anti-PD1 neutralizing antibody 1μg/ml (pembrolizumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.