Biosimilar Reference Antibodies
To develop a superior biologic drug, it is important to synthesize a benchmark reference antibody. The reference antibody can greatly facilitate the downstream development. It can help identify critical cell lines, reagents, performance characteristics, etc.
Strict Quality Control
Active Protein Detection
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human PD1, mFc-His tagged protein (PME100025) can bind Anti-PD-1 Neutralizing antibody in a linear range of 0.24-6.49 ng/ml.
Comparison of flow functional detection
Figure 2. HEK293 cell line transfected with irrelevant protein (B) and human PD1(A) were surface stained with anti-PD1 neutralizing antibody 1μg/ml (pembrolizumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.