DiTagTM pH-sensitive IgG labeling reagents
DiTagTM pH-sensitive IgG labeling reagents provide a convenient solution for measuring the internalization activities of antibodies. These reagents utilize a pH-sensitive fluorescently labeled Fc binding protein, which binds to IgG antibodies from various species, resulting in the formation of a fluorescently labeled antibody-reagent complex. After antibody internalization, the surrounding pH becomes acidic, significantly enhancing the fluorescence signal of the antibody-reagent complex. The fluorescence intensity serves as an indicator to determine the internalization activity of antibodies. By measuring the strength of the fluorescence signal, researchers can assess the efficiency of antibody internalization into cells. This information is crucial for understanding the cellular uptake mechanism of antibodies and assessing their efficacy in targeted therapies or diagnostic applications. Additionally, monitoring the fluorescence intensity can provide insights into the kinetics of antibody internalization, helping researchers optimize experimental conditions and improve the design of antibody-based drug delivery systems.