Nanodisc is a disc structure with a diameter around 10nm, which can keep transmembrane proteins in the environment of lipid bilayer, maintaining its conformation and biological functions.
Unlike other membrane scaffold protein (MSP) Nanodisc on the market, synthetic Nanodisc developed by DIMA Bio can be prepared directly from the mammalian cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified by affinity purification.
On-shelf Target Proteins
・Highly purified membrane proteins
・High solubility in aqueous solutions
・Proteins are in a native membrane environment and remain biologically active
・No detergent and can be used for cell-based assays
・No MSP backbone proteins
・SPR affinity analysis
・Phage display screening
・Cell based assays
・Protein crystal structure analysis
Human MDR-1 full length protein-synthetic nanodisc (FLP100029) 12 transmembrane protein
Human MDR-1-Nanodisc, Flag Tag on SDS-PAGE
MDR-1-Nanodisc (FLP100029) can bind anti-Flag monoclonal antibody and the EC50 is 6.883ng/ml.
Human GPRC5D full length protein-synthetic nanodisc (FLP100011) 7 transmembrane protein
Human GPRC5D-Nanodisc, Flag Tag on SDS-PAGE
GPRC5D-Nanodisc (FLP100011) can bind anti-GPRC5D mAb (DME100090) and the EC50 is 32.86ng/ml.
Human CLDN6 full length protein-synthetic nanodisc (FLP100008) 4 transmembrane protein
Human CLDN6-Nanodisc, Flag Tag on SDS-PAGE
CLDN6-Nanodisc (FLP100008) can bind anti-CLDN6 mAb (BME100082) and the EC50 is 66.99ng/ml.