Synthetic Nanodisc

Nanodisc is a disc structure with a diameter around 10nm, which can keep transmembrane proteins in the environment of lipid bilayer, maintaining its conformation and biological functions.
Unlike other membrane scaffold protein (MSP) Nanodisc on the market, synthetic Nanodisc developed by DIMA Bio can be prepared directly from the mammalian cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified by affinity purification.

    Nanodisc Platform

    On-shelf Target Proteins

    The diagram of nanodisc

    Advantages:
    ・Highly purified membrane proteins
    ・High solubility in aqueous solutions
    ・High stability
    ・Proteins are in a native membrane environment and remain biologically active
    ・No detergent and can be used for cell-based assays
    ・No MSP backbone proteins

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    Applications:
    ・ELISA
    ・SPR affinity analysis
    ・Phage display screening
    ・Immunization
    ・Cell based assays
    ・Protein crystal structure analysis

    Case Studies

    Human MDR-1 full length protein-synthetic nanodisc (FLP100029) 12 transmembrane protein

    Human GPRC5D full length protein-synthetic nanodisc (FLP100011) 7 transmembrane protein

    Human CLDN6 full length protein-synthetic nanodisc (FLP100008) 4 transmembrane protein

    Full length protein-synthetic nanodisc

    TargetCatalog NumberProduct Name
    CLDN6FLP100008Human CLDN6 full length protein-synthetic nanodisc
    GPRC5DFLP100011Human GPRC5D full length protein-synthetic nanodisc
    SSTR2FLP100013Human SSTR2 full length protein-synthetic nanodisc
    CLDN18.2FLP100014Human CLDN18.2 full length protein-synthetic nanodisc
    ADORA2AFLP100020Human ADORA2A full length protein-synthetic nanodisc
    TM4SF1FLP100022Human TM4SF1 full length protein-synthetic nanodisc
    CB1FLP100023Human CB1 full length protein-synthetic nanodisc
    CCR4FLP100024Human CCR4 full length protein-synthetic nanodisc
    OR2H1FLP100025Human OR2H1 full length protein-synthetic nanodisc
    CD20FLP100027Human CD20 full length protein-synthetic nanodisc
    CCR2FLP100028Human CCR2 full length protein-synthetic nanodisc
    MDR-1FLP100029Human MDR-1 full length protein-synthetic nanodisc
    CD63FLP100030Human CD63 full length protein-synthetic nanodisc
    GPR75FLP100031Human GPR75 full length protein-synthetic nanodisc
    SLC25A4FLP100032Human SLC25A4 full length protein-synthetic nanodisc
    TRPA1FLP100033Human TRPA1 full length protein-synthetic nanodisc
    TSPAN33FLP100035Human TSPAN33 full length protein-synthetic nanodisc
    F2RL1FLP100036Human F2RL1 full length protein-synthetic nanodisc
    CCR8FLP100037Human CCR8 full length protein-synthetic nanodisc