Clone ID | DMC446 |
---|---|
Target | |
Synonyms | C-Kit; CD117; MASTC; PBT; SCFR; KIT |
Host Species | Rabbit |
Description | Anti-CD117 antibody(DMC446); IgG1 Chimeric mAb |
Delivery | In Stock |
Uniprot ID | P10721 |
IgG type | Rabbit/Human Fc chimeric IgG1 |
Clonality | Monoclonal |
Reactivity | Human |
Applications | Flow Cyt |
Recommended Dilutions | Flow Cyt 1:100 |
Purification | Purified from cell culture supernatant by affinity chromatography |
Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains; a transmembrane region; and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand; stem cell factor (SCF); this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation; differentiation; migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis; stem cell maintenance; gametogenesis; melanogenesis; and in mast cell development; migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors; mast cell disease; acute myelogenous leukemia; and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. |
Usage | Research use only |
Conjugate | Unconjugated |
Anti-CD117 antibody(DMC446), IgG1 Chimeric mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. Flow cytometry analysis with Anti-CD117 (DMC446) on Expi293 cells transfected with human CD117 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).
Figure 2. Flow cytometry analysis of antigen binding of anti-human CD117 mAb(DMC100446).
(A) DMC100446 does not bind to A431 cells that do not express CD117.
(B) A clear peak shift of DMC100446 was seen compared to the control when incubated with CD117-expressing TT cells, indicating strong binding of DMC100446 to CD117. Antibodies were incubated at 5 μg/mL.
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