Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

Blog

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The List of ECD Proteins

sp-pme100625b fcrl5 sp1

ECD Proteins

Biotinylated Human FCRL5 Protein, His Tag

SKU:  PME100625B     Target:  FCRL5     Tag:  C-6×His Avi Tag

Expression Host:  HEK293

Price:10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme f100001b il31 sp1

ECD Proteins

Biotinylated Feline IL31 Protein, His Tag

SKU:  PME-F100001B     Target:  IL31     Tag:  C-6×His Avi Tag

Expression Host:  HEK293

Price:10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme d100004b il31 sp1

ECD Proteins

Biotinylated Canine IL31 Protein, His Tag

SKU:  PME-D100004B     Target:  IL31     Tag:  C-6×His Avi Tag

Expression Host:  HEK293

Price:10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100781b cd19 sp1

ECD Proteins

Biotinylated Human CD19 Protein, mFc Tag

SKU:  PME100781B     Target:  CD19     Tag:  C-Mouse Fc Avi tag

Expression Host:  HEK293

Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme d100006b pd1 sp1

ECD Proteins

Biotinylated Canine PD-1 Protein, mFc Tag

SKU:  PME-D100006B     Target:  PD1     Tag:  C-Mouse Fc Avi tag

Expression Host:  HEK293

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme100840b il7ra sp1

ECD Proteins

Biotinylated Human IL7RA Protein, hFc Tag

SKU:  PME100840B     Target:  IL7RA     Tag:  C-Human Fc Avi tag

Expression Host:  HEK293

Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101558b cd19 sp1

ECD Proteins

Biotinylated Human CD19(M75V,R76S,F85S) Protein, hFc Tag

SKU:  PME101558B     Target:  CD19     Tag:  C-Human Fc Avi tag

Expression Host:  HEK293

Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101051b fgl1 sp1

ECD Proteins

Biotinylated Human FGL1 Protein, hFc Tag

SKU:  PME101051B     Target:  FGL1     Tag:  N-Avi Human Fc tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme100025b pd 1 sp1

ECD Proteins

Biotinylated Human PD-1 Protein, mFc-His tag

SKU:  PME100025B     Target:  PD-1     Tag:  C-Mouse Fc and 6×His Avi tag

Expression Host:  HEK293

Price:10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100054b 4 1bb ligand sp1

ECD Proteins

Biotinylated Human 4-1BB Ligand Protein, mFc-His Tag

SKU:  PME100054B     Target:  4-1BB Ligand     Tag:  N-Mouse Fc and C-6×His Avi tag

Expression Host:  HEK293

Price:10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100022b ox40 sp1

ECD Proteins

Biotinylated Human OX40 Protein, hFc-His tag

SKU:  PME100022B     Target:  OX40     Tag:  C-Human Fc and 6×His Avi tag

Expression Host:  HEK293

Price:10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme c100119 cd4 sp1

ECD Proteins

Cynomolgus CD4 Protein, His Tag

SKU:  PME-C100119     Target:  CD4     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme c100118 cd2 sp1

ECD Proteins

Cynomolgus CD2 Protein, His Tag

SKU:  PME-C100118     Target:  CD2     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme c100117 cd98 sp1

ECD Proteins

Cynomolgus CD98 Protein, His Tag

SKU:  PME-C100117     Target:  CD98     Tag:  N-10×His tag

Expression Host:  HEK293

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme c100116 lgr5 sp1

ECD Proteins

Cynomolgus LGR5 Protein, hFc Tag

SKU:  PME-C100116     Target:  LGR5     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme c100115 cd8a sp1

ECD Proteins

Cynomolgus CD8A Acidic tail Protein, His Tag

SKU:  PME-C100115     Target:  CD8A     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme c100114 lilrb4 sp1

ECD Proteins

Cynomolgus LILRB4 Protein, His Tag

SKU:  PME-C100114     Target:  LILRB4     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme c100113 il12b sp1

ECD Proteins

Cynomolgus IL12B Protein, His Tag

SKU:  PME-C100113     Target:  IL12B     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme c100112 il4ra sp1

ECD Proteins

Cynomolgus IL4RA Protein, His Tag

SKU:  PME-C100112     Target:  IL4RA     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme c100111 cd5 sp1

ECD Proteins

Cynomolgus CD5 Protein, His Tag

SKU:  PME-C100111     Target:  CD5     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme c100110 epha2 sp1

ECD Proteins

Cynomolgus EPHA2 Protein, His Tag

SKU:  PME-C100110     Target:  EPHA2     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme c100109 cd161 sp1

ECD Proteins

Cynomolgus CD161 Protein, hFc Tag

SKU:  PME-C100109     Target:  CD161     Tag:  N-Human Fc tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme m100141 lilrb4 sp1

ECD Proteins

Mouse LILRB4 Protein, His Tag

SKU:  PME-M100141     Target:  LILRB4     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme m100140 il12b sp1

ECD Proteins

Mouse IL12B Protein, His Tag

SKU:  PME-M100140     Target:  IL12B     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme m100139 cd2 sp1

ECD Proteins

Mouse CD2 Protein, His Tag

SKU:  PME-M100139     Target:  CD2     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme m100138 mouse igg2a fc sp1

ECD Proteins

Mouse IgG2a-Fc Protein, Tag Free

SKU:  PME-M100138     Target:  IgG1-Fc     Tag:  No tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101980 siglec8 sp1

ECD Proteins

Human SIGLEC8 Protein, His Tag

SKU:  PME101980     Target:  SIGLEC8     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101979 pmel sp1

ECD Proteins

Human PMEL(25-595) Protein, hFc Tag

SKU:  PME101979     Target:  PMEL     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101977 lrp1 sp1

ECD Proteins

Human LRP1(3332-3779) Protein, hFc Tag

SKU:  PME101977     Target:  LRP1     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101976 lrp1 sp1

ECD Proteins

Human LRP1(786-1165) Protein, hFc Tag

SKU:  PME101976     Target:  LRP1     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101975 lrp1 sp1

ECD Proteins

Human LRP1(2522-2941) Protein, hFc Tag

SKU:  PME101975     Target:  LRP1     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101974 trfe sp1

ECD Proteins

Human TRFE Protein, His Tag

SKU:  PME101974     Target:  TRFE     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00