Extracellular Domain (ECD) Protein
Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.
Features and Applications
Features
Used as lmmunogen for
Therapeutic mAbs
Different Animal Model Species and Fragments
Validated in Therapeutic
mAbs Functional Screening
For Immunization
ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.
Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.
Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).
For Protein Activity
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.
Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.
Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.
HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
Hot Druggable Targets
Immune checkpoint proteins
Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.
Hematological cancer targets
Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.
More hematological cancer
Solid tumor targets
Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.
More solid tumor targetsBlog
The List of ECD Proteins
ECD Proteins
SKU: PME-C100133 Target: TSLP Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00
ECD Proteins
SKU: PME-C100132 Target: PMEL Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME-C100131 Target: PODXL Tag: C-10×His tag
Expression Host: HEK293
Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00
ECD Proteins
SKU: PME-C100130 Target: ALB Tag: C-10×His tag
Expression Host: HEK293
Price:10μg $91.00; 50μg $328.00 ; 100μg $488.00
ECD Proteins
SKU: PME-M100143 Target: HGF Tag: C-3×Flag Tag
Expression Host: HEK293
Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME-M100142 Target: ALB Tag: C-10×His tag
Expression Host: HEK293
Price:10μg $91.00; 50μg $328.00 ; 100μg $488.00
ECD Proteins
SKU: PME102017 Target: CCL2 Tag: C-3×Flag Tag
Expression Host: HEK293
Price:10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00
ECD Proteins
SKU: PME102016 Target: IL33 Tag: C-6×His tag
Expression Host: HEK293
Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00
ECD Proteins
SKU: PME102015 Target: FCRL5 Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $79.00; 50μg $281.00 ; 100μg $422.00
ECD Proteins
SKU: PME102013 Target: FCRL5 Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00
ECD Proteins
SKU: PME102012 Target: FCRL5 Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00
ECD Proteins
SKU: PME102011 Target: TSLP Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00
ECD Proteins
SKU: PME102010 Target: IGF-1R Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00
ECD Proteins
SKU: PME102009 Target: IGF-1R Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00
ECD Proteins
SKU: PME102008 Target: IGF-1R Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00
ECD Proteins
SKU: PME102007 Target: IGF-1R Tag: C-10×His tag
Expression Host: HEK293
Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00
ECD Proteins
SKU: PME102004 Target: CXCR3 Tag: C-Mouse Fc tag
Expression Host: HEK293
Price:10μg $79.00; 50μg $281.00 ; 100μg $422.00
ECD Proteins
SKU: PME102003 Target: PODXL Tag: C-10×His tag
Expression Host: HEK293
Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00
ECD Proteins
SKU: PME102002 Target: CD19 Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00
ECD Proteins
SKU: PME102001 Target: CD19 Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00
ECD Proteins
SKU: PME102000 Target: CD7 Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $72.00; 50μg $272.00; 100μg $409.00
ECD Proteins
SKU: PME101999 Target: CD7 Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $72.00; 50μg $272.00; 100μg $409.00
ECD Proteins
SKU: PME101998 Target: IL1RAP Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00
ECD Proteins
SKU: PME101996 Target: IL1RAP Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00
ECD Proteins
SKU: PME101994 Target: BRD4 Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $78.00; 50μg $310.00 ; 100 μg $460.00
ECD Proteins
SKU: PME101993 Target: BRD4 Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $78.00; 50μg $310.00 ; 100 μg $460.00
ECD Proteins
SKU: PME-C100129 Target: CLEC9A Tag: N-Human Fc tag
Expression Host: HEK293
Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00
ECD Proteins
SKU: PME-C100128 Target: TSLP Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00
ECD Proteins
SKU: PME-C100127 Target: IL22 Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $78.00; 50μg $310.00 ; 100 μg $460.00
ECD Proteins
SKU: PME-C100126 Target: IL17A Tag: C-Human Fc tag
Expression Host: HEK293
Price:10μg $91.00; 50μg $328.00 ; 100μg $488.00
ECD Proteins
SKU: PME-C100125 Target: IL1RL2 Tag: C-10×His tag
Expression Host: HEK293
Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00
ECD Proteins
SKU: PME-C100124 Target: EPCAM Tag: C-10×His tag
Expression Host: HEK293
Price:10μg $72.00; 50μg $272.00; 100μg $409.00