Nanodiscs

What are Nanodiscs?

Nanodiscs are essential for membrane protein (MP) research, overcoming challenges in expression, purification, and functional testing. Full-length multi-pass membrane proteins are difficult to express and often misfold or form inclusion bodies, resulting in low yields and instability. Traditional detergents destabilize these proteins, making structural studies and functional assays more difficult.

Nanodiscs solve these issues by creating a stable, membrane-like environment that encapsulates membrane proteins in a near-native state. This platform enhances protein stability, activity, and structural integrity, making it ideal for structural analysis, functional studies, and drug screening. Formats like MSP, Synthetic, and PeptiNanodiscs each offer unique advantages, enabling more reliable membrane protein research and accelerating drug discovery.

DIMA BIOTECH's Types of Nanodiscs

As mentioned before, Nanodiscs can be classified based on the stabilizing agents used. The major formats are MSP Nanodiscs, Synthetic Nanodiscs, and PeptiNanodiscs. To fully leverage Nanodiscs, DIMA BIOTECH uses the HEK293 system to produce recombinant membrane proteins that closely mimic their natural forms, supporting faster and more reliable drug discovery. Currently, DIMA BIOTECH has rich experience in developing full-length multi-pass membrane proteins with Synthetic Nanodiscs and PeptiNanodiscs.
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Synthetic Nanodisc

A Polymer-Based Solution for Multipass Transmembrane Proteins, Covering 500+ Targets Included in GPCR, Ion Channel, Transportors etc.

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PeptiNanodiscs

A Peptide-Based Solution for Multipass Transmembrane Proteins, Stabilizing Membrane Proteins for Cell-Based Applications.

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Validation Data of Nanodisc-based MPs

The purity of Human SLC7A11-Nanodisc (FLP100048) is greater than 90% as determined by SEC-HPLC.

Human GPR75-synthetic nanodisc (FLP120031) can bind Loaded Human GPR75 mAb (DMC100368) with an affinity constant of 5.02nM.

Human CLDN18.2-synthetic nanodisc (FLP120014) can bind loaded Human CLDN18.2 mAb (BME100075) with an affinity constant of 8.09nM.

GPRC5D PeptiNanodisc (Cat.No.FLP400011) and G4S-PE protein were used to detect the positivity rate of GPRC5D CAR-T cells.

Citation

[1] Li J, Zhou Z, Wu Y, et al. Heat acclimation defense against exertional heat stroke by improving the function of preoptic TRPV1 neurons. Theranostics. 2025 Jan 1;15(4):1376-1398. Product from DIMA BIOTECH: Human TRPV1 full length protein-synthetic nanodisc (FLP100128)