| Clone ID | DM53 |
|---|---|
| Target | |
| Immunogen | PME100012 |
| Synonyms | B7-H3; CD276; B7 homolog 3; B7H3 |
| Host Species | Rabbit |
| Description | Anti-B7-H3 antibody(DM53); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | Q5ZPR3 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Endotoxin | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage&Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Sterility | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| Background | The protein encoded by this gene belongs to the immunoglobulin superfamily; and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors; the protein is preferentially expressed only in tumor tissues. Additionally; it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA; and there is an inverse correlation between the expression of this protein and miR29 levels; suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Usage | Research use only |
| Conjugate | Unconjugated |
| DIMA Disclaimer | All DIMA recombinant antibodies are genuinely generated by DIMA Biotech. They are all under patent application. Any protein sequencing or reverse engineering attempt is prohibited. We are actively scr |
Anti-B7-H3 antibody(DM53); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Flow cytometry data of serially titrated Rabbit anti-B7H3 monoclonal antibody (clone: DM53) on on HEK293 cell line transfected with human B7-H3. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 2. HEK293 cell line transfected with irrelevant protein (A) and human B7-H3 (B) were surface stained with Rabbit anti-B7-H3 monoclonal antibody 1μg/ml (clone: DM53) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
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