| Tag | C-Flag Tag |
|---|---|
| Target | |
| Synonyms | N/A |
| Description | Cyno AQP5 full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | A0A2K5V4J8 |
| Expression Host | HEK293 |
| Protein Families | N/A |
| Protein Pathways | N/A |
| Molecular Weight | The cyno full length AQP5 protein has a MW of 28.3 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage&Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient. Plays an important role in fluid secretion in salivary glands. Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells. Seems to play a redundant role in water transport in the eye, lung and in sweat glands. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Cyno AQP5 full length protein-synthetic nanodisc
Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag Tag AQP5-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with AQP5-nanodisc is 4.374ng/ml.
Figure 2. Cyno AQP5-Nanodisc, Flag Tag on SDS-PAGE
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