| Clone ID | DM100 |
|---|---|
| Target | |
| Synonyms | CD40; Bp50; CDW40; MGC9013; TNFRSF5; p50 |
| Host Species | Rabbit |
| Description | Anti-CD40 antibody(DM100); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P25942 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt; WB; IHC |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100; WB 1:1000; IHC 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching; memory B cell development; and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand; which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation; and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Usage | Research use only |
Anti-CD40 antibody(DM100), Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD40 protein, mFc-His tagged protein PME100015 can bind Rabbit anti-CD40 monoclonal antibody ( clone: DM100) in a linear range of 0.64-80 ng/ml.
Figure 2. A. Flow cytometry analysis with Anti-CD40 ( DM100) on Expi293 cells transfected with human CD40 ( Blue histogram) or Expi293 transfected with irrelevant protein ( Red histogram).
Figure 3. Flow cytometry analysis of antigen binding of rabbit anti-human CD40 mAb(DME100100).
(A) DME100100 does not bind to Jurkat cells that do not express CD40.
(B) A clear peak shift of DME100100 was seen compared to the control when incubated with CD40-expressing Raji cells, indicating strong binding of DME100100 to CD40. Antibodies were incubated at 2 μg/mL.
Figure 4. Anti-CD40 antibody (SKU# DME100100) at 1/1000 dilution
Lane : Raji (human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 30 kDa
Observed band size: 45 kDa
Figure 5. A. DME100100 at 10µg/ml staining CD40 in Raji cells by IHC (SKU# DME100100);B. DME100100 at 10µg/ml staining CD40 in human tonsil tissue by IHC (SKU# DME100100)