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elisa-FLP100017 CLDN18.2 Fig.1 Elisa 1

Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP)

Price: 25test $180.00 ; 100test $530.00

SKU: FLP100017 Category:
Product Data
Synonyms

Claudin 18.2
Uniprot ID

P56856
Expression Host

HEK293
Description

Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP)
Molecular Weight

783bp
Delivery

3~4 weeks
Formulation & Reconstitution

Supplied in 1xPBS (pH 7.4)
Package

25test $180.00 ; 100test $530.00
Storage & Shipping

The product should be stored at -80°C. Please do not repeated freeze-thaw cycles.
Background

The protein encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2010]
Images

elisa-FLP100017 CLDN18.2 Fig.1 Elisa 1
Figure1. ELISA plates were pre-coated with 0.5μg/per well purified human CLDN18.2-EGFP-VLP. Serial diluted Anti-CLDN18.2 monoclonal antibody (BME100075) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for anti- CLDN18.2 monoclonal antibody binding with CLDN18.2-EGFP-VLP is 877.5ng/ml.
fc-FLP100017 CLDN18.2 Fig.2A FC 1fc-FLP100017 CLDN18.2 Fig.2B FC 1fc-FLP100017 CLDN18.2 Fig.2C FC 1fc-FLP100017 CLDN18.2 Fig.2D FC 1
Figure2. FACS analysis of CLDN18.2 VLP A. Negative Control 1: Expi293 cells were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP).
B. Negative Control 2: Anti-GPRC5D-CAR-Expi293 cells (an irrelevant CAR) were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP).
C. Positive Control: Anti-CLDN18.2-CAR-Expi293 cells were stained with biotin labeled Protein L, followed by streptavidin-APC antibody.
D. Anti-CLDN18.2-CAR-Expi293 cells were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP).