| Target | |
|---|---|
| Synonyms | CD3-DELTA;IMD19;T3D and IMD18;T3E;TCRE |
| Description | Recombinant Human CD3D Protein with C-terminal 6×His tag and Human CD3E Protein with C-terminal human Fc tag |
| Delivery | In Stock |
| Uniprot ID | P04234;P07766 |
| Expression Host | HEK293 |
| Tag | C-6×His Tag and C-Human Fc Tag |
| Molecular Characterization | CD3D(Phe22-Ala105) 6×His tag and CD3E(Asp23-Asp126) hFc(Glu99-Ala330) |
| Molecular Weight | The protein has a predicted molecular mass of 10.4 and 37.9 kDa after removal of the signal peptide. The apparent molecular mass of CD3D-His and CD3E-hFc is approximately 35-55 kDa due to glycosylation. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | T-cell surface glycoprotein CD3 delta and CD3 epsilon chain, also known as CD3D and CD3E or CD3DandCD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. |
| Usage | Research use only |
Human CD3D Protein, His Tag and Human CD3E Protein, hFc Tag
Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00
Product Data
Images
Figure 1. Human CD3D and CD3E Heterodimer Protein, His Tag and hFc Tag on SDS-PAGE under reducing condition.