Recombinant human CD70 protein with N-terminal mouse Fc and C-terminal 6×His tag
N-Mouse Fc and C-6×His Tag
mFc(Pro99-Lys330) CD70(Gln39-Pro193) 6×His
The protein has a predicted molecular mass of 45.6 kDa after removal of the signal peptide.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
|Formulation & Reconstitution||
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
|Storage & Shipping||
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis.
Research use only
Human CD70 Protein, mFc-His Tag
Price: 10μg $93.00 ; 50μg $370.00 ; 100 μg $555.00
Figure 1. Human CD70 Protein, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD27, hFc tagged protein (PME100475) can bind Human CD70,mFc-His tagged protein (PME100042) in a linear range of 3.2-400 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) CD70,mFc-His tagged protein (PME100042) can bind Anti-CD70 Neutralizing antibody (BME100005) in a linear range of 3.91-139.9 ng/ml.
Figure 4. Flow cytometry analysis with 15 μg/mL Human CD70 Protein, mFc-His tag (PME100042) on Expi293 cells transfected with human CD27 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).