| Tag | C-Flag&Strep Tag |
|---|---|
| Expression Host | HEK293 |
| Target | |
| Synonyms | CD223 |
| Description | Human LAG3-Strep full length protein-synthetic nanodisc |
| Uniprot ID | P18627 |
| Protein Families | Transmembrane |
| Protein Pathways | N/A |
| Molecular Weight | The human full length LAG3-Strep protein has a MW of 57.4kDa |
| Delivery | In Stock |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Sterility | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| Storage&Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human LAG3-Strep full length protein-synthetic nanodisc
Price: 10 μg $810.00 ; 50 μg $3960.00 ; 100 μg $7200.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag LAG3-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-LAG3 monoclonal antibody (BME100062) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-LAG3 monoclonal antibody binding with LAG3-Strep-Nanodisc is 337.6ng/ml.
Figure 2. Human LAG3-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
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