Brain-Derived Neurotrophic Factor; BDNF; Abrineurin
Recombinant Human Brain-Derived Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding His129-Arg247 is expressed.
E.coli expression system
Greater than 95% as determined by reducing SDS-PAGE.
|Formulation & Reconstitution||
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.
|Storage & Shipping||
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Research use only
Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. Along with other structurally related neurotrophic factors NGF, NT-3 and NT-4, BDNF binds with high affinity to the TrkB kinase receptor. It also binds with the LNGFR (for low-affinity nerve growth factor receptor, also known as p75). BDNF promotes the survival, growth and differentiation of neurons. It serves as a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. BDNF expression is altered in neurodegenerative disorders such as Parkinson's and Alzheimer's disease.
Figure 1. Greater than 95% as determined by reducing SDS-PAGE.
Figure 2. Immobilized Human TrkB-His at 2ug/ml (100 μl/well) can bind Human BDNF*: Biotinylated by NHS-biotin prior to testing. The ED50 of Human BDNF* is 47.58 ng/ml.