CMT1F; CMT2E; CMTDIG; NF-L; NF68; NFL; PPP1R110
|Storage & Shipping||
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
|Formulation & Reconstitution||
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Recombinant Human NEFL(9-88) with C-terminal human Fc tag
The protein has a predicted molecular mass of 34.9 kDa after removal of the signal peptide. The apparent molecular mass of NEFL(9-88)-hFc is approximately 35-55 kDa due to glycosylation.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Research use only
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
Human NEFL (9-88) Protein, hFc Tag
Price: 10μg $83.00 ; 50μg $333.00 ; 100 μg $500.00
SKU: PME100654 Category: ECD Proteins
Figure 1. Human NEFL(9-88) Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human NEFL (9-88) Protein, hFc Tag (PME100654) can bind Anti-NEFL(9-88) antibody(DM198), Rabbit mAb (DME100198) in a linear range of 0.128-80 ng/mL.