Recombinant Human ROR1 Protein with C-terminal 6×His tag
ROR1(Gln30-Glu403) 6×His tag
The protein has a predicted molecular mass of 42.8 kDa after removal of the signal peptide. The apparent molecular mass of ROR1-His is approximately 55-70 kDa due to glycosylation.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
|Formulation & Reconstitution||
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
|Storage & Shipping||
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2012]
Research use only
Human ROR1 Protein, His Tag
Price: 10μg $80.00 ; 50μg $319.00 ; 100 μg $478.00
Figure 1. Human ROR1 Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human ROR1, His tagged protein (PME100399) can bind Anti-ROR1 Neutralizing antibody (BME100073) in a linear range of 0.64-16 μg/ml.
Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human ROR1 Protein, His Tag (PME100399) can bind Anti-ROR1(NBE 002 biosimilar) mAb (BME100191) in a linear range of 0.64–16 ng/mL.