| Target | |
|---|---|
| Synonyms | Siglec-8, SAF-2, SIGLEC8, SAF2 |
| Description | Recombinant human SIGLEC8 Protein with C-terminal 6×His tag |
| Delivery | In STOCK |
| Uniprot ID | Q9NYZ4 |
| Expression Host | HEK293 |
| Tag | C-6×His tag |
| Molecular Characterization | SIGLEC8(Met17-Ala363)+6×His tag |
| Molecular Weight | The protein has a predicted molecular mass of 38.6 kDa after removal of the signal peptide. |
| Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Sterility | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| Storage&Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized proteins are shipped at ambient temperature. |
| Background | SIGLEC8 (Sialic acid-binding immunoglobulin-like lectin 8) is an inhibitory cell-surface receptor belonging to the CD33-related Siglec family. It is predominantly expressed on human eosinophils, mast cells, and basophils, where it recognizes specific sialylated glycans and regulates immune-cell activation. The cytoplasmic region of SIGLEC8 contains immunoreceptor tyrosine-based inhibitory motifs that recruit signaling molecules and suppress downstream cellular responses. Engagement of SIGLEC8 can induce apoptosis in eosinophils and inhibit mast-cell activation, making it an important regulator of allergic inflammation and eosinophil-associated diseases. SIGLEC8 has therefore emerged as a promising therapeutic target for conditions such as asthma, chronic rhinosinusitis with nasal polyps, eosinophilic disorders, and mast-cell-mediated diseases. Recombinant SIGLEC8 protein is suitable for glycan-binding studies, receptor–ligand interaction assays, antibody screening, drug discovery, and research on allergic and eosinophilic inflammation. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human SIGLEC8 Protein, His Tag
Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Human SIGLEC8 Protein, His Tag on SDS-PAGE under reducing condition.
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