| Target | |
|---|---|
| Synonyms | DIF;TNF-alpha;TNFA;TNFSF2;cachexin;cachectin;TNFα |
| Description | Recombinant human TNFa protein with C-terminal 6×His tag |
| Delivery | In Stock |
| Uniprot ID | P01375 |
| Expression Host | HEK293 |
| Tag | C-6×His Tag |
| Molecular Characterization | TNFα(Arg78-Leu233) 6×His tag |
| Molecular Weight | The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human TNFa Protein, His Tag
Price: 10μg $91.00; 50μg $348.00 ; 100 μg $522.00
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Figure 1. Human TNFα Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2.ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human TNFa , His tagged protein (PME100076) can bind Anti-TNFa Neutralizing antibody (BME100056) in a linear range of 0.64-16 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TNFα Protein, His Tag (PME100076) can bind Anti-TNFa(Certolizumab biosimilar) mAb (BME100183) in a linear range of 0.64–16 ng/mL.
Figure 4. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TNFα Protein, His Tag (PME100076) can bind Anti-TNFa(golimumab biosimilar) mAb (BME100179) in a linear range of 3.20–80 ng/mL.
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