Recombinant human TNFa protein with C-terminal 6×His tag
TNFa(Val77-Leu233) 6×His tag
The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
|Formulation & Reconstitution||
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
|Storage & Shipping||
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Research use only
Human TNFa Protein, His Tag
Price: 10μg $87.00 ; 50μg $348.00 ; 100 μg $522.00
Figure 1. Human TNFα Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human TNFa , His tagged protein (PME100076) can bind Anti-TNFa Neutralizing antibody (BME100056) in a linear range of 0.64-16 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TNFα Protein, His Tag (PME100076) can bind Anti-TNFa(Certolizumab biosimilar) mAb (BME100183) in a linear range of 0.64–16 ng/mL.
Figure 4. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TNFα Protein, His Tag (PME100076) can bind Anti-TNFa(golimumab biosimilar) mAb (BME100179) in a linear range of 3.20–80 ng/mL.