Recombinant mouse CD40L protein with N-terminal human Fc tag
N-Human Fc Tag
hFc(Glu99-Ala330) Mouse CD40 Ligand(Gly115-Leu260)
The protein has a predicted molecular mass of 42.1 kDa after removal of the signal peptide. The apparent molecular mass of hFc-CD40L is approximately 40-55 kDa due to glycosylation.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
|Formulation & Reconstitution||
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
|Storage & Shipping||
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (By similarity). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (PubMed:1374165).[UniProtKB/Swiss-Prot Function]
Research use only
Mouse CD40 Ligand Protein, hFc Tag
Price: 10μg $100.00 ; 50μg $375.00 ; 100 μg $550.00
SKU: PME-M100068 Category: ECD Proteins
Figure 1. Mouse CD40 Ligand Protein, hFc Tag on SDS-PAGE under reducing condition.