| Tag | N-Flag, C-Strep Tag |
|---|---|
| Target | |
| Synonyms | N/A |
| Description | Negative control-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | N/A |
| Expression Host | HEK293 |
| Protein Families | N/A |
| Protein Pathways | N/A |
| Molecular Weight | The protein has a MW of 42.8 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping | Store at -20'C to -80'C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliguot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.Protect from light. |
| Background | Negative control-synthetic nanodisc can be used as isotype control for nanodisc displaying membrane proteins in various applications. The protein in the nanodisc is a fusion protein, divided into three parts. N-terminal extracellular region is Flag tag. C-terminal intracellular region is EGFP-Strep tag. The transmembrane region of this protein utilizes the transmembrane region of platelet-derived growth factor receptor beta (PDGFRB). |
| Usage | Research use only |
| Conjugate | Fluorescent |
Negative control-synthetic nanodisc
Price: 10 μg $630.00 ; 50 μg $3080.00 ; 100 μg $5600.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Elisa plates were added with serial diluted N-Flag, C-Strep Tag Negative control-Nanodisc on an anti-Flag monoclonal antibody pre-coated (0.2μg/per well) plate. Strep tactin-HRP (1:5000) was used as detection antibody. From above data, the EC50 for Strep tactin-HRP binding with Negative control-Nanodisc is 283.0ng/ml.
Figure 2. Negative control-Nanodisc, N-Flag, C-Strep Tag on SDS-PAGE
Figure 3. WB analysis of Negative control-Nanodisc with Strep-Tactin HRP at 1/4000 dilution
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