Tag | C-Flag&Strep Tag |
---|---|
Target | |
Synonyms | BLT1; BLTR; CMKRL1; GPR16; LTB4R1; LTBR1; P2RY7; P2Y7 |
Description | Human LTB4R-Strep full length protein-synthetic nanodisc |
Delivery | In Stock |
Uniprot ID | Q15722 |
Expression Host | HEK293 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | Neuroactive ligand-receptor interaction |
Molecular Weight | The human full length LTB4R-Strep protein has a MW of 37.6 kDa |
Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | A member of the rhodopsin subfamily of G-protein-coupled receptors that is expressed in the pancreas and gastrointestinal tract. The encoded protein is activated by lipid amides including lysophosphatidylcholine and oleoylethanolamide and may be involved in glucose homeostasis. This protein is a potential drug target in the treatment of type 2 diabetes. |
Usage | Research use only |
Conjugate | Unconjugated |
Human LTB4R-Strep full length protein-synthetic nanodisc
Price: 10 μg $810.00 ; 50 μg $3960.00 ; 100 μg $7200.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag LTB4R-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with LTB4R-Strep-nanodisc is 1.636ng/ml.
Figure 2. Human LTB4R-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
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